The research group led by Guangsheng YANG from National Key Laboratory of Crop Genetic Improvement of Huazhong Agricultural University has made a further progress on the research of rapefertility regulative mechanism. They successfully cloned a new specific brassica gene MS5. The relative result has been published online on the journalPlant Cell.

It is known that using hybrid isone of the important way to increase the rape yield. And the nucleus malesterility (CMS) is often used for hybrid seed production. The Yi3A was an archetypal CMS found in China. Through a genetic analysis, it has revealed thatthe fertility of Yi3A was controlled by 3 multiple all eles on MS5 locus.

This time, the researcher has cloned the MS5 successfully and analyzed the function of it combined with the research team led by Zhukuan CHENG from Institute of Genetics and Developmental Biology of Chinese Academy of Sciences. The research showed that MS5 encoded a specific brassica protein (orphan gene) and regulated the development of germ cell through regulating the early chromo some structure in meiosis and meiosis process.

The further study has showed that the function exertion of this gene depended on the expression quantity of its function protein. In a single dose of insufficient manner, it led to the genotype MS5bMS5c be CMS and made the 3 multiple alleles of MS5 present absolute recessive relation.

Some relative experts said that the cloning of this gene has not only enhanced the people’s understanding of early meiosis process, but also has laid the theoretical basis for the effective use of this gene.

Source: China Science Daily

Time: 2016.5.26